Dynamics of Ultrastructural Changes of Ileal Cells After Ligation of the Right Colic Artery
Introduction. Surgical treatment of stenosing diseases of the esophagus remains a challenging and unsolved problem. This is evidenced by the large number of complications, high figures of postoperative mortality, which ranges from 3.5 to 30 %. There is no consensus about optimal way of performing esophageal replacement in the world (stomach, small or large intestine used for esophageal replacement). So we can see the search for new methods of esophageal replacement. We proposed a new method of esophageal replacement that can be used for simultaneous impression of the esophagus and stomach, where sufficient conditions have been established blood supply to the graft, would be an opportunity to extend the graft to the required size, kept antireflux mechanism and function of artificial stomach. This is achieved by holding of esophageal replacement by ileocecal segment. Therefore, we studied the ultrastructural changes in the wall of the ileocecal segment after ligation of appropriate feeding arteries in experiment, which is carried out at its mobilization for esophageal replacement.
The aim of our study was to investigate the dynamics of ultrastructural changes in the cells of the ileum of rats after ligation of right colic artery in 7, 14 and 21 days.
Material and Methods. The experiment was carried out on white male rats, weighing from 250 to 300g. Experiments were performed in accordance with general principles of experiment on animals adopted by the National Congress of Bioethics. Before research animals passed quarantine in a vivarium for of week, contained in identical conditions, received an identical diet. There was not any special preoperative preparation. In total 16 rats were operated. Operations were performed under ketamine anesthesia. The ligation of right colic artery with the subsequent studying of ultrastructural changes of a wall of ileum was carried out in all animals of study group (10 rats). There were 6 rats in control group. Animals were taken out of experience in 7, 14, 21 days by an overdose of ketamine and biopsy specimens were performed to study the ultrastructural changes in the cells of the ileum.
Results and Discussion. Electronic and microscopic study showed the development of degenerative changes of all epithelial, smooth muscle cells and endothelial cells of capillaries of the ileum in 7 days after ligation of the right colic artery, and also there was violation of the transcellular transport of substances, water and electrolytes.
In subsequent periods the signs of activation of intracellular metabolism were revealed, they manifested in a hyperplasia of granular endoplasmic reticulum membranes, appearance dividers forms of mitochondria and increasing of the number of ribosomes and polysomes.
Conclusion. By 21 days after ligation of the right colic artery the submicroscopic structure of all epithelial cells, smooth muscle cells and endothelial cells of capillaries of the ileum is completely restored.
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